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A genetic screen for mutations affecting embryogenesis in zebrafish. Segregation analyses and gene-centromere distances in zebrafish. Streisinger, G., Singer, F., Walker, C., Knauber, D. Replication, integration and stable germ-line transmission of foreign sequences injected into early zebrafish embryos. Efficient recovery of ENU-induced mutations from the zebrafish germline. Induction of recessive lethal and specific locus mutations in the zebrafish with ethyl nitrosourea. Induction of mutations by γ-rays in pregonial germ cells of zebrafish embryos. Frequency of γ-ray induced specific locus and recessive lethal mutations in mature germ cells of the zebrafish, Brachydanio rerio. Production of clones of homozygous diploid zebra fish ( Brachydanio rerio). Streisinger, G., Walker, C., Dower, N., Knauber, D. The biology and use of zebrafish, Brachydanio rerio in fisheries research. Observations of the early development of the zebrafish.
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A gene-driven ENU-based approach to generating an allelic series in any gene. A gene-driven approach to the identification of ENU mutants in the mouse. Suppressor screen in Mpl −/− mice: c-Myb mutation causes supraphysiological production of platelets in the absence of thrombopoietin signaling.
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Efficient gene-driven germ-line point mutagenesis of C57BL/6J mice. Genome-wide, large-scale production of mutant mice by ENU mutagenesis. A systematic, genome-wide, phenotype-driven mutagenesis programme for gene function studies in the mouse. Zebrafish are an emerging model for therapeutic drug discovery by 'chemical genetics', whereby chemical libraries are screened to find small molecules that suppress disease-related phenotypes. Zebrafish are proving to be good models of several acquired diseases including cancer, inflammation and infection. Transgenic zebrafish disease models with tissue-restricted disease-gene expression and fluorescently marked cell types enable disease mechanisms to be studied in vivo.
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Reverse-genetic techniques such as TILLING (targeting induced local lesions in genomes) allow the recovery of zebrafish with mutant disease-gene alleles, overcoming the lack of a technique for gene targeting by homologous recombination. Large-scale chemical and insertional mutagenic screens have generated many zebrafish mutants with defects that are analogous to human genetic diseases, at the molecular and cellular-pathological levels. Genetic resources include a genome-sequencing project that is nearing completion. Genetic techniques that have been developed for zebrafish make them an attractive vertebrate model for efficient, cost-effective, invertebrate-style forward genetics. Particularly useful attributes of zebrafish include: ex vivo fertilization and embryogenesis optical transparency of embryos and larvae rapid embryological development cheap housing costs and genetic tractability. The area is dealing with its driest period in at least 1,200 years.Zebrafish have long contributed to biological and medical research, especially as a model for developmental and toxicological studies. These droughts - exacerbated by climate change - continue to disrupt the West. Las Vegas began pumping for its water supply from deeper in the lake because of how depleted the reservoir became, the Associated Press reported in May. Lake Mead has hit its lowest water levels since 1937 and is filled to 27% of capacity, according to NASA. The newest discovery of human remains found in the lake comes as the reservoir suffers from an ongoing 22-year-long drought. The investigation is still ongoing and the cause of death has not been identified. Reports emerged about another set of remains found at Swim Beach, according to authorities. The cause of death in this case remains unknown. Rouse said this set was more skeletal than the previous remains found, which had organ tissue, CNN also reported. Clark County Coroner Melanie Rouse believes the remains were from a person between the approximate ages of 23 and 37, according to CNN. Two sisters found skeletal remains at Callville Bay. Climate Change Is Making It Worse May 7, 2022: Callville Bay Environment The Drought In The Western U.S.